MicroscopyView.com

MICRO MEASURING 

How to mesure the HFOV ?

For trying this for the first time the simple way is :

Now you can take the picture of the specimen 

after taking a good picture of a specimen take another one of a scale.

and make a proportional resample of :  4267 x 3200 pixels.

If the objective is 40x or more use a stage micrometer of 1 mm

If the objective is 2x, 4x or 10x you can use a micrometer of 10 mm

design for eyepiece. It is better if your scale cover all the horizontal

view of the picture.

Never crop the picture of the specimen before making mesurement.

With a software like Photoshop make a high resample

of the picture of the scale  by  8000 x 6000 pixels.

Crop the scale image for showing a round number of divisions :

10 divisions = 0.1 mm    or    20 divisions = 0.2 mm

Now look on the property of the image for see the number of pixels

for the width. If the picture of your specimen was taken with a 40x

you will obtain about 3750 pixels for 20 divisions or for 0.2 mm.

    Crop picture

3750 pixels = 0.2 mm    then    8000 pixels = 0.4266 mm

    20 divisions : 0.2 mm

The HFOV  for this lens in this particular condition is :  427 μm

    3750 pixels